We recommend that you upgrade your browser. The following is a list of popular options:
The rationale for the Methylasse project is four-fold:
Bio-conversion of methane avoids shortcomings assoicated with chemical methane conversion.
De novo construction of a methylase is a novle approach and departs from earlier efforts in engineering methanotroph metabolsim and in improving biological Methane-to-Liquid (MTL) fuel production.
As opposed to the methane monooxygenases (MMOs) in methanotrophs, the constructed methylase is intended to operate without consumption of reducing power.
The objectives in METHYLASE are to:
Employ rational protein engineering in combination with accelerated evolution to convert an existing carboxylase to a methylase enzyme.
Develop a high-throughput assay for Methylase activity.
Incorporate the Methylase into a novel Methane Assimilation (M-A) metabolic cycle that will allow for assimilatio of CH4 into Acetyl-CoA a fuel intermediate.