Earth Sciences Division (ESD) Department of Energy (DOE) Lawrence Berkeley National Laboratory (LBNL)


In this project, we study carbohydrate metabolism in barley, sorghum, rice, and other cereals [1-8]. Of particular interest is the regulation of sugar biosynthesis in endosperm and stem tissues. We uncovered the first reported transcription factors, the SUSIBAs (for SUgar SIgnaling in BArley) involved in sugar signaling and regulation of starch biosynthesis in the barley endosperm [6, 7]. We have identified SUSIAB2 orthologs in wheat, corn, rice and sorghum, and have proposed a model for how the SUSIBAs control starch biosyntheis via sugar signaling (Fig. 1). We are now launching a project where we investigate how the sorghum SUSIBA2 ortholog controls carbon partiitoning of sugars to the stem in sweet and grain varieties of sorghum. This effort is a collaboration with Chuanxin Sun at the Swedish University of Agricultural Sciences and Peggy Lemaux at UC Berkeley.

References

[1] Mangelsen, E., Wanke, D., Kilian. J., Sundberg, E., Harter, K., and Jansson, C. (2010) Significance of light, sugar, and amino acid supply for diurnal gene regulation in developing barley caryopses. Plant Physiol. 153, 14-33.

[2] Mutisya, J., Sun, C., and Jansson, C. (2009) Circadian oscillation of starch branching enzyme gene expression on the sorghum endosperm. Plant Sign. Behavior 4, 871-872.

[3] Mutisya, J., Sun, C., Rosenquist, S., and Jansson, C. (2009) Endogenous oscilation of SBE expression in sorghum endosperm. J. Plant Physiol. 166, 428-434.

[4] Mangelsen, E., Kilian, J., Berendzen, K.W., Kolukisaoglu, Ü., Harter, K., Jansson, C., and Wanke, D. (2008) Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots. BMC Genomics 9, doi:10.1186/1471-2164-9-194.

[5] Mutisya, J., Sun, C., Rosenquist, S., Baguma, Y., Odhiambo, B., and Jansson, C. (2006) Transcriptional regulation of the sbeIIb genes in sorgum (Sorghum bicolor) and barley (Hordeum vulgare): importance of the barley sbeIIb second intron. J. Plant Physiol. 163, 770-780.

[6] Sun, C., Olsson, H., Höglund, A.-S., Mangelsen, E. and Jansson, C. (2005) Antisense oligodeoxynucleotide inhibition as a potent strategy in plant biology: identification of SUSIBA2 as a transcriptional activator in plant sugar signaling. Plant J. 44, 128-138. (Selected for Cover Image).

[7] Sun, C., Ahlandsberg, S., Palmqvist, S., Ohlsson, H, Borén, M. and Jansson, C. (2003) A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the SURE element of the iso1 promoter. Plant Cell 15, 2076-2092.

[8] Mutisya, J., Sathish, P., Sun, C., Andersson, L., Ahlandsberg, S., Baguma, Y., Palmqvist, S., Odhiambo, B., Åman, P. and Jansson, C. (2003) Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression. J. Plant Physiol. 160, 921-930.

See a complete publication list.